Indian J Exp Biol. 2003 Mar;41(3):238-41.

Wound healing property of ethanolic extract of leaves of Hyptis suaveolens with supportive role of antioxidant enzymes.

Shirwaikar A, Shenoy R, Udupa AL, Udupa SL, Shetty S.

Department of Pharmacognosy, College of Pharmaceutical Sciences, Manipal 576119, India. annieshirwaikar@yahoo.com

Ethanolic extract of leaves of Hyptis suaveolens was evaluated for its wound healing activity in ether-anaesthetized Wistar rats at two different doses (400 and 800 mg/kg) using incision, excision, and dead space wound model. Significant increase in skin breaking strength, granuloma breaking strength, wound contraction, hydroxyproline content and dry granuloma weight and decrease in epithelization period was observed. A supportive study made on granuloma tissue to estimate the levels of catalase and superoxide dismutase recorded a significant increase in the level of these antioxidant enzymes. Granuloma tissue was subjected to histopathological examination to determine the pattern of lay-down for collagen using Van Gieson and Masson Trichrome stains. Enhanced wound healing activity may be due to free radical scavenging action of the plant and enhanced level of antioxidant enzymes in granuloma tissue. Better collagenation may be because of improved antioxidant studies.